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The Biacore 3000 is biosensor instrument that relies on surface plasmon resonance to monitor changes in the refractive index of the medium near the surface of a glass/gold chip. When a ligand is fixed to the gold chip surface the binding of analytes flowing over the chip surface can describe the kinetics of association and dissociation. The binding reactions are followed by an optical bench on the opposite side of the chip that measures the binding-associated changes in refractive index by monitoring the surface plasmon resonance. Each chip has 4 binding surfaces that can be followed simultaneously. Software is available to derive kon and koff and calculate affinity constants for several models. Affinity constants can be measured at various temperatures and this data can be used to calculate enthalpy by the van’t Hoff equation. Equilibrium experiments can also be performed with this instrument.
Room 302 Goss Laboratories
1925 Coffey Road
The current business model for Biacore use is to recover costs for a service contract (currently about $14,000 per year). We total the usage and bill users based on the portion of the time they have used. We normally have approximately 100 to 120 days of use each year; this leads to bills that charge approximately $100+ per day of use. Bills will arrive around March 1.
Contact & scheduling
Hoepf [dot] 1 [at] osu [dot] edu
Prior to using the instrument investigators must undergo training. New users are required to read the following materials:
- Survey of the 1998 optical biosensor literature
- Improving biosensor analysis
- Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensors, Karlsson, R. and Falt, A.
- Guidelines for regeneration
- Workshop Series-immobilization chemistries and sensor chips, Biasymposium 1999, San Francisco.
Folders containing hard copies of these articles are available in room 302 of Goss Laboratory.
New User Education
All new users must undergo training or provide convincing evidence of past training. Training begins with reading the papers listed above. After one has read this literature, an instrument training session is scheduled. Investigators must purchase a Biacore Starter Kit (catalogue # BR-1003-19) and a CM-5 chip (catalogue # BR-1000-14) that will be used during training. After training is completed the investigator can schedule time on the instrument. Scheduling is performed through a calendar in 302 Goss. Scheduling is done in one-day blocks. Scheduled time can be cancelled up to 24 hours prior to the reservation.
Investigators will be charged for time they schedule, including time that is not used.
Surface Plasmon Resonance Links
Surface plasmon resonance references
- Methods in Enzymology 240, 323-349 (1994)